Differentiation of primate pluripotent stem cells to...

Details Differentiation of primate pluripotent stem cells to... Differentiation of primate pluripotent stem cells to...

C - Chemistry, Metallurgy

12

N

C12N 5/077 (2010.01) C12N 5/0735 (2010.01)

Patent

CA 2611809

The present application describes the new methods for the differentiation of primate pluripotent stem cells into cardiomyocyte-lineage cells. The methods utilize sequential culturing of the primate pluripotent stem cells in certain growth factors to produce cardiomyocyte-lineage cells. In certain embodiments of the invention, the population of cells produced by the sequential culturing is further enriched for cardiomyocyte-lineage cells so as to produce a higher percentage of those cells.

L'invention décrit de nouveaux procédés de différenciation de cellules souches pluripotentes primates en des cellules à lignage cardiomyocyte. Les procédés consistent à utiliser la mise en culture séquentielle des cellules souches pluripotentes primates de certains facteurs de croissance pour produire des cellules à lignage cardiomyocyte. Dans certains modes de réalisation de l'invention, la population cellulaire produite par la mise en culture séquentielle est encore enrichie en cellules à lignage cardiomyocyte de manière à produire une pourcentage plus élevé de ces cellules.

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