Differentiation of stem cells to pancreatic endocrine cells

Details Differentiation of stem cells to pancreatic endocrine cells Differentiation of stem cells to pancreatic endocrine cells

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/0735 (2010.01) A61K 35/39 (2006.01) C12Q 1/02 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2435826

A method is provided for differentiating embryonic stem cells to endocrine cells. The method includes generating embryoid bodies from a culture of undifferentiated embryonic stem cells, selecting endocrine precursor cells, expanding the endocrine precursor cells by culturing endocrine cells in an expansion medium that comprises a growth factor, and differentiating the expanded endocrine precursor cells in a differentiation media to differentiated endocrine cells produced by this method are also provided. Artificial islets are disclosed, as well as method for using the pancreatic endocrine cells and the artificial islets.

L'invention concerne un procédé permettant de différentier les cellules souches embryonnaires des cellules endocrines. Ce procédé consiste à générer des corps embryoïdes à partir d'une culture de cellules souches embryonnaires non différentiées, à sélectionner les cellules précurseurs endocrines, à accroître les cellules précurseurs endocrines en cultivant les cellules endocrines dans un milieu d'expansion qui comprend un facteur de croissance, et à différencier ces cellules précurseurs endocrines ayant subi une expansion dans des milieux de différentiation par rapport aux cellules endocrines différentiées. L'invention traite aussi de cellules endocrines du pancréas produites par ce procédé. L'invention a aussi pour objet des îlots pancréatiques et des îlots artificiels.

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