Dimeric and trimeric nucleic acid dyes, and associated...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07D 213/02 (2006.01) C07D 401/02 (2006.01)

Patent

CA 2601455

Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.

La présente invention concerne des colorants d'acides nucléiques dimériques et trimériques ainsi que des systèmes et procédés associés. Un tel colorant peut former une structure en épingle à cheveux qui lui permet de colorer des acides nucléiques, par exemple par le biais d'un mécanisme de libération sur demande. Un tel colorant peut présenter une faible fluorescence de fond en l'absence d'acides nucléiques et une forte fluorescence en présence d'acides nucléiques, lors de sa liaison avec ceux-ci, par exemple. Un colorant de cette invention peut être utilisé dans une multitude d'applications, telles que dans l'analyse quantitative d'ADN dans une réaction en chaîne de la polymérase (PCR) en temps réel.

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