Diphenylhydantoin immunogens

Details Diphenylhydantoin immunogens Diphenylhydantoin immunogens

C - Chemistry, Metallurgy

07

G

167/139, 530/17.

C07G 7/00 (1980.01)

Patent

CA 1142511

ABSTRACT OF THE DISCLOSURE Reagents for use in binding assays, particularly immuno- assays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including .beta.-galactosyl-unbelliferone- diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N1, N3 and .sigma.-phenyl derivatives of diphenylhydantoin for preparing same. N1 and N3-.omega.- aminoalkyl and .sigma.-(.omega.-aminoalkoxy)- phenyl derivatives are prepared and coupled by amide linkage to a .beta.-galactosyl-unbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N1 and N3-.omega.-carboxyalkyl and .sigma.-(.omega.-carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic poly- amino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.

393747

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