Direct assay of free analyte

Details Direct assay of free analyte Direct assay of free analyte

G - Physics

01

N

150/11, 167/40

G01N 23/00 (2006.01) G01N 33/78 (2006.01)

Patent

CA 1113391

DIAGNOSTIC METHOD Inventors: George H. Parsons, Jr. Stephen M. Sherrill ABSTRACT OF THE DISCLOSURE Free analytes in samples containing free analytes and receptor- bound analytes are determined by absorbing at least a portion of free analyte from the sample with insolubilized receptor or receptor which is then made insoluble, washing the insoluble receptor, adding labelled sample analyte or labelled sample analyte receptor, incubating, washing the insoluble phase free of the soluble phase and determining the label in either phase. The method solves a long standing need in the assay of free thyroid hormones.

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