Direct assay of skin protein in skin samples removed by tape...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/60 (2006.01) A61B 10/02 (2006.01) C12Q 1/00 (2006.01) G01N 1/04 (2006.01) G01N 1/30 (2006.01)

Patent

CA 2608884

The present invention provides for a method of measuring the amount of skin removed by tape stripping. In one aspect of the invention, the invention provides a method for the direct assay of protein in skin samples removed by tape stripping, with a view to combining the protein measurement obtained with a corresponding skin cholesterol measurement to identify individuals at risk of having atherosclerosis as well as those at risk of developing atherosclerosis and similar diseases associated with and attributable to high cholesterol levels. Moreover, the present invention allows a comparative measurement of the amount of skin removed by tape stripping that does not rely solely on the area of the sample removed. Additionally, in one aspect of the invention, the method of this invention can allow relative levels of skin cholesterol to be compared based on the relative amounts of skin removed.

La présente invention concerne un procédé de mesure de la quantité de peau prélevée par un pelliculage à bande. Dans un aspect de l'invention, ladite invention concerne un procédé de dosage direct d'une protéine dans des échantillons de peau prélevés par pelliculage à bande, de façon à combiner la mesure de protéine obtenue avec une mesure de cholestérol cutané correspondante afin d'identifier des individus présentant un risque d'athérosclérose ainsi que ceux présentant un risque de développer une athérosclérose ainsi que des maladies similaires associées à des niveaux de cholestérol élevés et imputables à ceux-ci. De plus, la présente invention permet une mesure comparative de la quantité de peau prélevée par pelliculage à bande ne reposant pas uniquement sur la zone de l'échantillon prélevé. De plus, dans un aspect de l'invention, le procédé de cette invention peut permettre de comparer des niveaux de cholestérol cutané relatifs sur la base des quantités relatives de peau prélevées.

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