Direct identification and mapping of rna transcripts

Details Direct identification and mapping of rna transcripts Direct identification and mapping of rna transcripts

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2559401

The present invention provides methods for determining the sequence of a population of RNA transcripts generated from a DNA sample prepared from a cell population. The method comprises the steps of providing a DNA sample from a population of cells and preparing the DNA fragments such that each fragment has only one RNA polymerase promoter. The DNA fragments are transcribed using a wild type RNA polymerase to obtain RNA transcripts, the sequence of which are determined using RNA sequencing or hybridization techniques.

L'invention concerne des méthodes pour déterminer la séquence d'une population de transcrits d'ARN générés à partir d'un échantillon d'ADN prélevé dans une population de cellules. La méthode comprend une étape de préparation d'un échantillon d'ADN prélevé dans une population de cellules et une étape de préparation des fragments d'ADN de sorte que chaque fragment contienne uniquement un promoteur d'ARN polymérase. Les fragments d'ADN sont transcrits au moyen d'une polymérase d'ARN de type sauvage afin de produire des transcrits D'ARN dont la séquence est déterminée par des techniques de séquençage ou d'hybridation d'ARN.

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