Direct molecular cloning of a modified eukaryotic...

Details Direct molecular cloning of a modified eukaryotic... Direct molecular cloning of a modified eukaryotic...

C - Chemistry, Metallurgy

12

N

C12N 15/863 (2006.01) C12N 7/01 (2006.01) C12N 15/39 (2006.01) C12N 15/54 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2617830

The invention provides a plasmid for producing a modified chordopoxvirus, wherein the plasmid comprises a segment of a chordopoxvirus genome that comprises a thymidine kinase gene of said chordopoxvirus, wherein said thymidine kinase gene has been modified to prevent expression of active thymidine kinase, wherein said plasmid is as shown in Figure 4.2 and is selected from the group of plasmids: pHindJ-2 comprising the sequence of SEQ. ID. NO. 4, and pHindJ-3 comprising the sequence of SEQ. ID. NO. 5.

L'invention concerne un plasmide pour produire un chordopoxvirus modifié. Le plasmide comprend un segment d'un génome de chordopoxvirus qui contient un gène de thymidine kinase dudit chordopoxvirus. Le gène de thymidine kinase a été modifié pour empêcher l'expression d'une thymidine kinase active. Le plasmide en question (figure 4.2) est choisi parmi le groupe de plasmides suivants : pHindJ-2 comprenant la séquence de SEQ. ID. NO. 4 et pHindJ-3 comprenant la séquence de SEQ. ID. NO. 5.

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