Direct multiplex characterization of genomic dna

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2426824

The invention is directed to novel methods of multiplexing nucleic acid reactions, including amplification, detection and genotyping. The invention relies on the use of percircle probes that are circularized in the presence of the corresponding target nucleic acids, cleaved, and then amplified.

La présente invention concerne de nouveaux procédés de multiplexage de réactions d'acides nucléiques, telles que l'amplification, la détection et le génotypage. Cette invention repose sur l'utilisation de sondes précurseurs de cercles qui sont circularisées en présence des acides nucléiques cibles correspondants, segmentées puis amplifiées.

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