C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/44 (2006.01) C12Q 1/48 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2543033
The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.
L'invention concerne des méthodes et des routines médicales permettant de développer et d'optimiser des essais de détection d'acide nucléique destinés à être utilisés dans la recherche fondamentale, la recherche clinique, et pour le développement d'essais de détection clinique. L'invention porte notamment sur des méthodes de conception d'amorces oligonucléotidiques destinées être utilisées dans des réactions d'amplification multiplex. L'invention se rapporte aussi à des méthodes d'optimisation de réactions d'amplification multiplex, ainsi qu'à des méthodes d'essais de génération de signaux et de cibles combinés.
Allawi Hatim T.
Gerber Chad
Hall Jeff G.
Jarvis Nancy
Kurensky David
Smart & Biggar
Third Wave Technologies Inc.
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