Direct radioimmunoassay for antigens and their antibodies

G - Physics – 01 – N

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G01N 33/48 (2006.01) G01N 23/00 (2006.01) G01N 33/576 (2006.01)

Patent

CA 1059901

A B S T R A C T A method for direct radioimmunoassay of antigens or their associated antibodies utilizing a coated test apparatus comprising adding a serum to be tested for an antigen to test apparatus coated with its antibody; incu- bating the tubes for from 0.5 to 42 hours; aspirating the contents and washing the same with a Tris-HCl and sodium azide mixture; adding purified I125 labeled antibody into the tube and incubating for from 1 to 24 hours; aspirating and washing the contents; and counting the time for I125 radiation, A similar assay for the antibody may be con- ducted utilizing an antigen coated apparatus and I125 labeled antigen. The method for coating these apparatus such as tubes comprises adding the antigen or antibody solution in a Tris-HCl, sodium azide solution into a suitable test tube or well, incubating the tubes at room temperature, aspirating and washing the contents and storing at from between 2 and 25° C. until use.

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