Direct snp detection with unamplified dna

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 1/00 (2006.01)

Patent

CA 2508359

The present invention provides methods for detecting a target nucleic acid molecule in a sample that comprises nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules. In particular, the present invention provides methods and probes for detecting a single nucleotide polymorphism (SNP) in a sample that comprises nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules.

La présente invention se rapporte à des procédés permettant de détecter une molécule d'acide nucléique cible dans un échantillon qui contient des molécules d'acide nucléique dont la complexité biologique est supérieure à celle de molécules d'acide nucléique amplifiées. En particulier, l'invention concerne des procédés et des sondes permettant de détecter un polymorphisme à nucléotide unique (SNP) dans un échantillon qui contient des molécules d'acide nucléique dont la complexité biologique est supérieure à celle de molécules d'acide nucléique amplifiées.

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