C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2421963
We describe a method of selecting an enzyme having replicase activity, the method comprising the steps of: (a) providing a pool of nucleic acids comprising members each encoding a replicase or a variant of the replicase; (b) subdividing the pool of nucleic acids into compartments, such that each compartment comprises a nucleic acid member of the pool together with the replicase or variant encoded by the nucleic acid member; (c) allowing nucleic acid replication to occur; and (d) detecting amplification of the nucleic acid member by the replicase. Methods for selecting agents capable of modulating replicase activity, and for selecting interacting polypeptides are also disclosed.
L'invention se rapporte à un procédé de sélection d'une enzyme ayant une activité de réplicase. Ledit procédé consiste: (a) à utiliser un ensemble d'acides nucléiques comportant des éléments codant chacun une réplicase ou un variant de réplicase; (b) à diviser l'ensemble d'acides nucléiques en compartiments de sorte que chaque compartiment comprenne un élément acide nucléique de l'ensemble ainsi que la réplicase ou un variant codé par l'élément acide nucléique; (c) à permettre la réplication de l'acide nucléique; et (d) à détecter l'amplification de l'élément acide nucléique par la réplicase. L'invention se rapporte également à des procédés permettant de sélectionner des agents capables de moduler l'activité de réplicase et permettant également de sélectionner des polypeptides d'interaction.
Ghadessy Farid
Holliger Philipp
Ong Jennifer Lee
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Medical Research Council
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