Directed genetic modifications of human stem cells

Details Directed genetic modifications of human stem cells Directed genetic modifications of human stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 5/02 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2515108

Human embryonic stem cells can be genetically transformed by a combination of electroporation and homologous recombination. This technique makes it possible to create targeted inserts or deletions to the genome of the stem cells. This ability makes it possible to create populations of progeny cells which have differentiated into a target cell type of a specific desired lineage.

L'invention concerne des cellules souches humaines que l'on peut transformer génétiquement par combinaison d'électroporation et de recombinaison homologue. Cette technique permet de créer des inserts ciblés ou des délétions au niveau du génome des cellules souches. Cette capacité permet de créer des populations de cellules progénitrices différenciées en type de cellules cibles d'une lignée spécifique recherchée.

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