Discontinuous countercurrent chromatographic process and...

G - Physics – 01 – N

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G01N 30/44 (2006.01) B01D 15/08 (2006.01)

Patent

CA 2238954

A preparative chromatographic cyclical process comprising, at steady state: establishing a figure-of-eight circulating chromatographic profile between two chromatographic columns with the provision that said chromatographic profile never passes through a pump; discontinuously and periodically injecting a sample comprising at least two components, into said circulating profile; and collecting, discontinuously and periodically, at least two enriched fractions from said circulating profile, the apparatus for conducting this process comprising only two chromatographic columns, at least one solvent pump, and injection pump, two 4-way valves, and conduit communicating said columns and the two 4-way valves in the form of a figure-of-eight pattern without traversing a pump, and conduit communicating said at least one solvent pump and at least one of said columns.

Divulgation d'un procédé cyclique de chromatographie de préparation consistant, à un état stable, à : établir un profil chromatographique de circulation en forme de huit entre deux colonnes chromatographiques avec la disposition que le profil chromatographique en question ne passe jamais à travers une pompe; l'injection discontinue et périodique d'un échantillon comprenant au moins deux composantes dans le profil en circulation; et la collecte discontinue et périodique d'au moins deux fractions enrichies du profil en circulation. L'appareil utilisé dans ce procédé ne comporte que deux colonnes de chromatographie, est doté d'au moins une pompe à solvant, d'une pompe d'injection, de deux soupapes à 4 voies et d'une conduite reliant les colonnes susmentionnées et les soupapes à 4 voies dans un motif en forme de huit sans traverser de pompe et une conduite communiquant avec au moins une pompe à solvant et au moins une des colonnes.

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