Discontinuous probe design using hybritope mapping

Details Discontinuous probe design using hybritope mapping Discontinuous probe design using hybritope mapping

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C12N 9/22 (2006.01)

Patent

CA 2207011

This invention provides a method of detecting or determining a binding oligonucleotide comprising a nucleotide sequence which binds within a known nucleotide sequence of a target nucleic acid using a technique called hybritope mapping. This invention also provides a method of using hybritope mapping to obtain discontinuous probes that bind to a target nucleic acid.

Procédé qui permet de détecter ou de déterminer un oligonucléotide de liaison comprenant une séquence nucléotidique qui se lie dans une séquence nucléotidique connue d'un acide nucléique cible, à l'aide d'une technique appelée cartographie hybritope. La présente invention concerne également un procédé d'utilisation de la cartographie par hybritope pour obtenir des sondes discontinues qui se lient à un acide nucléique cible.

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