Dispersed metal particle labelled assay

G - Physics – 01 – N

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G01N 33/74 (2006.01) G01N 33/553 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1135183

ABSTRACT OF THE DISCLOSURE The present invention relates to a method for the detection and/or determination of one or more components of the reaction between a specific binding protein and the correspond- ing bindable substance, in which one or more labelled compo- nents are used, that are obtained by coupling particles of a dispersion of a metal, metal compound or polymer nuclei, coated with a metal or metal compound, having a particle size of at least 5 nm, directly or indirectly to the desired component of said reaction. During the reaction or after an adequate reaction time, the physical properties and/or the amount of the metal and/or the formed metal containing agglomerate, is/are deter- mined in the test sample, or optionally after a separation of the bound and free metal-labelled components in one of the derived fractions by methods known by themselves.

331816

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