Displacement assay on a porous membrane

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/558 (2006.01)

Patent

CA 2241324

Displacement assays, under non-equilibrium conditions, are performed by flowing a liquid sample through a membrane (12) having binding elements with binding sites saturated with a labelled form of the analyte. Analyte in the sample displaces, under non-equilibrium conditions, the labelled form of the analyte from the membrane. The displaced labelled form of the analyte may then be detected.

On procède à des dosages du type à déplacement dans des conditions de non équilibre en faisant passer un échantillon liquide par une membrane (12) présentant des éléments de fixation dans des sites de fixation saturés en formes marquées de l'analyte. L'analyte de l'échantillon déplace, dans des conditions de non équilibre, la forme marquée de l'analyte à partir de la membrane. Il devient alors possible de détecter les formes marquées de l'analyte.

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