Displacement chromatography of proteins using low molecular...

C - Chemistry – Metallurgy – 07 – C

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C07C 217/08 (2006.01) B01D 15/42 (2006.01) C07C 215/40 (2006.01) C07K 1/18 (2006.01) G01N 30/02 (2006.01)

Patent

CA 2183160

A method for the purification of proteins by displacement chromatography on ion exchange media using molecular weight displacers is disclosed. Several classes of low molecular weight, charged species are exemplified, including aminoacids, peptides, antibiotics and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula (I), wherein R1 is lower alkyl, n is 2 to 6, and X is a common counter anion and similar dendritic polymers based thereon.

L'invention concerne un procédé de purification de protéines par chromatographie du déplacement sur des milieux à échange d'ions faisant intervenir des déplaceurs à faible masse moléculaire. Elle est illustrée à l'exemple de plusieurs catégories d'espèces chargées et de faible masse moléculaire, notamment des acides aminés, des peptides, des antibiotiques et des polymères dendrimères. Les nouveaux composés utilisables comme déplaceurs sont des dendrimères ayant la formule (I), dans laquelle R?1¿ représente alkyle inférieur, n est compris entre 2 et 6, et X est un contre-anion commun, ainsi que des polymères dendritiques similaires dérivés de ceux-ci.

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