Displacement polynucleotide assay method and polynucleotide...

Details Displacement polynucleotide assay method and polynucleotide... Displacement polynucleotide assay method and polynucleotide...

C - Chemistry, Metallurgy

12

Q

150/3, 150/8.5

C12Q 1/68 (2006.01)

Patent

CA 1247993

ABSTRACT DISPLACEMENT POLYNUCLEOTIDE ASSAY METHOD AND POLYNUCLEOTIDE COMPLEX REAGENT THEREFOR A diagnostic reagent is disclosed containing a com- plex of a probe polynucleotide (P) bound via purine/ pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) contains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the target nucleotide sequence (G) causes binding, initially between G and a single-stranded portion (IBR) of the target binding region (TBR). Thereafter the labeled polynucleotide (L) is displaced from the complex by branch migration of (G) into the (P)/(L) binding region. Determination of displaced labeled polynucleo- tide (L) gives a value which is a function of the presence and concentration of target nucleotide sequence (G) in the sample.

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