Disposable device in diagnostic assays

Details Disposable device in diagnostic assays Disposable device in diagnostic assays

G - Physics

01

N

G01N 33/53 (2006.01) B01L 3/00 (2006.01) G01N 33/543 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2156412

A disposable diagnostic device and method of its use are provided. The device comprises a housing containing first and second flow paths orthogonal to each other. The first flow path commences at a sample addition port and continues through a transport channel which feeds sample to an incubation area by means of capillary flow. The incubation area comprises a signal producing system and is underneath an optically-clear window. The first flow path terminates in a top waste reservoir which receives sample and wash fluid. The second flow path begins on one side of the incubation area at an inlet port over a side reagent reservoir. Liquid flows along the second flow path from the side reagent reservoir across the area into the side waste reservoir. The incubation area may comprise agitation means for homogenous dispersion of reagent into liquid. Various reagents of a signal producing system may be contained within the device and the necessary liquids added automatically by appropriate, so as to have the assay carried out automatically, without technician involvement, providing an accurate and sensitive determination.

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