Disposable tubing set for use with a cell expansion...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/26 (2006.01)

Patent

CA 2679097

A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves.

L'invention concerne un dispositif jetable d'expansion cellulaire, ayant au moins un bioréacteur. Le bioréacteur a une zone de croissance cellulaire et une zone d'alimentation, la zone de croissance cellulaire étant séparée de ladite zone d'alimentation par une membrane. Un trajet de recirculation de fluide en communication fluidique avec la zone de croissance cellulaire permet d'enlever hermétiquement un échantillon contenant de la matière cellulaire. Celui-ci peut comprendre un tube allongé ou une pluralité de segments de tube parallèles. Les segments de tube parallèles ont des extrémités d'entrée et des extrémités de décharge, et les extrémités d'entrée sont reliées au niveau d'une première jonction commune, et les extrémités de décharge sont reliées au niveau d'une seconde jonction commune. Les jonctions communes peuvent comprendre des clapets.

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