Dissociative fluorescence enhancement assay

Details Dissociative fluorescence enhancement assay Dissociative fluorescence enhancement assay

G - Physics

01

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G01N 33/542 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01) G01N 21/76 (2006.01) G01N 21/78 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2477385

The invention relates to an enhancement solution for an assay technology using lanthanide ions or their chelates as labels and dissociative fluorescence enhancement as a tool for detection, wherein said enhancement solution comprises a .szlig.-diketone of formula (I) wherein R1 is an aryl, optionally mono- or multi-substituted, and R2 is a straight or branched alkyl chain with 2 to 9 carbon atoms substituted with four or more fluorine atoms. The invention further relates to a bioaffinity assay using lanthanide ions or their chelates as labels and dissociative fluorescence enhancement as a tool for detection comprising the use of said enhancement solution.

L'invention concerne une solution d'amélioration pour une technologie de dosage utilisant des ions lanthanide ou leur chélates en tant qu'étiquettes et amélioration de fluorescence dissociative en tant qu'outil de détection, ladite solution d'amélioration contient une .szlig.-dicétone de la formule (I) dans laquelle R¿1? représente un aryle, facultativement monosubstitué ou multisubstitué, et R2 représente une chaîne alkyle droite ou ramifiée ayant 2 à 9 atomes de carbone substitués par 4 atomes de fluor ou d'avantage. L'invention concerne également un dosage de bioaffinité utilisant des ions lanthanide ou leurs chélates en tant qu'étiquettes et amélioration de fluorescence dissociative en tant qu'outil de détection, comprenant l'utilisation de ladite solution d'amélioration.

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