Distinguishing molecular forms

Details Distinguishing molecular forms Distinguishing molecular forms

G - Physics

01

N

G01N 33/53 (2006.01) C12Q 1/68 (2006.01) G01N 33/542 (2006.01)

Patent

CA 2415639

A method for detecting aggregation or polymerization of molecules in a sample, which method comprises the steps of: a) exposing a sample containing the molecules to at least two labelling moieties which will bind to only a single mutually exclusive site on each of the said molecules; and b) determining whether multiply-bound labelling moieties are present. The method is particularly of use in differentiating pathogenic aggregated protein molecules from non-pathogenic, non-aggregated protein molecules.

L'invention concerne un procédé de détection de l'agrégation ou de la polymérisation de molécules dans un échantillon, lequel procédé consiste : a) à exposer un échantillon contenant lesdites molécules à au moins deux fractions de marquage censées se lier uniquement à un seul site exclusif sur chacune des molécules ; et b) à déterminer s'il existe des fractions de marquage à plusieurs liaisons. Ledit procédé est particulièrement utile pour différencier des molécules de protéines pathogènes agrégées de molécules de protéines non pathogènes et non agrégées.

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