Dna and use thereof

Details Dna and use thereof Dna and use thereof

C - Chemistry, Metallurgy

12

N

530/13, 530/7.1,

C12N 15/12 (2006.01) C07K 14/47 (2006.01) C07K 14/575 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2019363

Disclosed are (1) a DNA containing a DNA segment coding for PACAP38; (2) a precursor protein of PACAP38; (3) a transformant containing a DNA having a DNA segment coding for PACAP38; (4) a method for preparing mature PACAP38 comprising cultivating the transformant described in the above (3), producing and accumulating a protein in a culture, and collecting the resulting protein; and (5) a method for preparing the above polypeptide comprising condensing a partial amino acid or a peptide which can constitute the mature PACAP38, with a residual portion, and removing a protective group if a product has the protective group. The DNA is applied to experimental animals to understand their brain functions, which serves to elucidate human brain functions. PACAP38 provides information about growth and maintenance of rat and human brain nerves, and can also be utilized as therapeutic agents for various neuropathy.

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