Dna assay for the prediction of autoimmune diabetes

Details Dna assay for the prediction of autoimmune diabetes Dna assay for the prediction of autoimmune diabetes

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2246487

The present invention relates to a novel DNA assay for the diagnosis and/or prediction of autoimmune diabetes. The present invention relates to a DNA assay for the prediction of autoimmune diabetes in human subjects, which comprises the steps of a) obtaining a DNA sample from the subject and PCR amplification of class III alleles of variable number of tandem repeats (VNTR) located upstream of the insulin gene (INS); and b) electrophoretic co-migration of amplified fragment to identify class III alleles of the insulin VNTR which are identical to S1 or S2, from alleles which differ from S1 and S2; whereby identification of S1 or S2 allele is indicative of at least 1% risk of autoimmune diabetes.

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