Dna chip comprising a microarray made of microelectrode systems

G - Physics – 01 – N

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G01N 33/543 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2510720

The invention relates to a DNA chip comprising a carrier (14) and a microarray of spots (1) containing immobilised catcher molecules which are arranged on said carrier. Each spot (1) contains a microelectrode system for the impedance spectroscopic detection of binding events occurring between the catcher molecules and target molecules of an analyte solution (38) applied to the spots (1). The microelectrode system has a pair of polarisation electrodes (2,4) in order to produce an alternating electromagnetic field and a pair of sensor electrodes (8,10) for measuring a voltage drop in the analyte (38).

L'invention concerne une puce à ADN qui comporte un support (14) ainsi qu'un microréseau de spots (1) contenant des molécules pièges immobilisées, placé sur ledit support. Chaque spot (1) contient un système de micro-électronique, servant à la détection, par spectroscopie d'impédance, d'événements de liaison entre les molécules piège et des molécules cibles d'une solution d'analyte (38) appliquée sur les spots (1). Le système de microélectrodes présente une paire d'électrodes de polarisation (2,4) servant à produire un champ électromagnétique alternatif et une paire d'électrodes de détection (8,10) servant à mesurer une chute de tension dans l'analyte (38).

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