Dna cloning vector plasmids and methods for their use

Details Dna cloning vector plasmids and methods for their use Dna cloning vector plasmids and methods for their use

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2542053

A group of cloning vector plasmids for use in constructing DNA molecules, such as transgenes, for the purpose of gene expression or analysis of gene expression. The present invention is also a method for using the cloning vector plasmids in a variable series of cloning steps to produce a final transgene product. The plasmid cloning vectors are engineered to minimize the amount of manipulation of DNA fragment components by the end user of the vectors and the methods for their use. Transgenes produced using the invention may be used in a single organism, or in a variety of organisms including bacteria, yeast, mice, and other eukaryotes with little or no further modification.

L'invention concerne un groupe de plasmides de vecteur de clonage que l'on utilise dans l'hybridation de molécules d'ADN, notamment des transgènes, en vue de l'expression génétique ou de l'analyse de l'expression génétique. La présente invention concerne également un procédé d'utilisation desdits plasmides dans des séries variables d'étapes de clonage afin d'obtenir un produit transgénétique final. Les vecteurs de clonage sont construits afin de minimiser la quantité de manipulation des composés de fragment d'ADN par l'utilisateur final des vecteurs et des procédés en vue de leur utilisation. Les transgènes produits au moyen de cette invention peuvent être utilisés dans un simple organisme ou dans une grande variété d'organismes, y compris des bactéries, de la levure, des souris et d'autres eucaryotes subissant une petite modification ou ne subissant aucune modification ultérieure.

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