Dna coding for a human vasoconstrictive peptide and use thereof

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 14/475 (2006.01) C07K 14/575 (2006.01) C12N 1/21 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2046754

TITLE: DNA CODING FOR A HUMAN VASOCONSTRICTIVE PEPTIDE AND USE THEREOF ABSTRACT OF THE DISCLOSURE Disclosed are (1) a DNA containing a cDNA segment coding for human endothelin-2 (SEQ ID NO:1), (2) a precursor of human endothelin-2 (SEQ ID NO:2), (3) a transformant carrying a DNA containing a cDNA segment coding for human endothelin-2, and (4) a method for preparing mature human endothelin-2 which comprises culturing the transformant described in (3), accumulating a protein in a culture medium, and collecting the same, whereby human endothelin-2 and the precursors thereof can be produced in large amounts.

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