Dna coding for human cell surface antigen

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) C07K 14/705 (2006.01) C07K 14/715 (2006.01) C07K 16/28 (2006.01) C12N 15/70 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2067031

The present invention is directed to DNAs coding for human cell surface antigen (Fas or Fas antigen), vectors for expressing said DNAs and transformants transfected with said vector. Fas is a polypeptide that exists in the surfaces of a variety of cells and is considered to be deeply concerned with the apoptosis of cells. The isolated Fas cDNA has an open reading frame that is capable of encoding a protein consisting of 335 amino acids. The mature Fas antigen is a protein consisting of 319 amino acids having a calculated molecular weight of about 36,000 and is constituted by an extracellular domain of 157 amino acids, a membrane-spanning domain of 17 amino acids, and a cytoplasmic domain of 145 amino acids.

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