Dna construct and its use

Details Dna construct and its use Dna construct and its use

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2382444

A DNA construct comprising in the 5' to 3' direction of transcription operably linked a promoter region directing transcription to the seed of an oilseed plant, a nucleotide sequence coding for at least one peptide with enzyme activity necessary for keto group containing xanthophyll production and esterification in an oilseed plant and a transcriptional termination region is disclosed. The DNA construct may additionally comprise a nucleotide sequence coding for a transit peptide directing the translated fusion polypeptide to the chloroplast of the oilseed plant. The peptide with enzyme activity is preferably a peptide with .beta.-carotene C-4-oxygenase activity, e.g. from the alga <aematococcus pluvialis. Comprised by the invention are also a transgenic oilseed plant cell, e.g. of rape, sunflower, soybean or mustard origin, and a transgenic oilseed plant-produced xanthophyll, such as canthaxanthin or astaxanthin, and also astaxanthin esters.

L'invention concerne une construction d'ADN comportant, dans la direction 5' vers 3' de la transcription, un site promoteur fonctionnellement lié orientant la transcription vers la semence d'une plante oléagineuse, une séquence nucléotidique codant pour au moins un peptide à activité enzymatique nécessaire à la production et l'estérification de groupe kéto contenant la xanthophylle dans une plante oléagineuse, ainsi qu'une région de terminaison transcriptionnelle. La construction d'ADN peut comporter également une séquence nucléotidique codant pour un peptide de transit orientant le polypeptide de fusion traduit vers le chloroplaste de la plante oléagineuse. De préférence le peptide à activité enzymatique est un peptide à activité de .beta.-carotène C-4-oxygénase, provenant de l'algue Haematococcus pluvialis. L'invention concerne en outre une cellule de plante oléagineuse transgénique, par exemple de colza, de tournesol, de soja ou de moutarde, ou de la xanthophylle produite par un plante oléagineuse, tel que canthaxanthine ou astaxanthine, ainsi que des esters d'astaxanthine.

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