Dna constructs containing a kluyveromyces alpha-factor...

Details Dna constructs containing a kluyveromyces alpha-factor... Dna constructs containing a kluyveromyces alpha-factor...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/81 (2006.01) C07K 14/39 (2006.01) C12N 9/64 (2006.01) C12N 15/11 (2006.01) C12N 15/59 (2006.01)

Patent

CA 1327762

ABSTRACT OF THE DISCLOSURE DNA constructs that are useful for providing secreto- ry expression of heterologous polypeptides in yeast compri- sing a DNA sequence that includes a Kluyveromyces .alpha.-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis .alpha.-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.

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