Dna constructs for specific inhibition of gene expression by...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/11 (2006.01)

Patent

CA 2627443

The invention relates to expression constructs and methods for their production which are suitable, after transfection thereof into eukaryotic cells, for inhibiting in a targeted manner in these cells the formation of defined proteins by RNA interference. Methods for producing such vectors contain no PCR steps, are a three-step procedure in one reaction vessel and can be carried out within a few hours. In addition, DNA expression constructs which are suitable for targeted inhibition of gene expression by means of RNA interference are, in addition, subject matter of the invention, wherein these are expression constructs which are also suitable for multiple gene expression inhibition.

L'invention concerne des produits de recombinaison d'expression et des procédés de fabrication de ces produits, lesdits produits permettant d'inhiber de façon ciblée par interférence ARN la formation de protéines définies dans des cellules eucaryotes après transfection desdits produits dans ces cellules. Les procédés de fabrication de ce type de vecteurs ne comportent pas d'étape de PCR, consistent en une procédure en trois étapes dans une cuve de réaction et peuvent être réalisés en peu de temps. L'invention concerne également des produits de recombinaison d'expression d'ADN permettant d'inhiber de façon ciblée l'expression de gènes par interférence ARN, lesdits produits de recombinaison d'expression d'ADN étant adaptés à de multiples inhibitions de l'expression de gènes.

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