Dna constructs for the expression of proteins in the mammary...

Details Dna constructs for the expression of proteins in the mammary... Dna constructs for the expression of proteins in the mammary...

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/89 (2006.01) A23C 9/00 (2006.01) C12N 5/10 (2006.01) C12N 9/64 (2006.01) C12N 15/18 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2040178

ABSTRACT OF THE DISCLOSURE A recombinant DNA construct for the expression of proteins in the mammary gland of transgenic mammals and the subsequent secretion into the milk, which construct contains the follow- ing DNA regions in the specified order and functionally linked to one another: (1) a transcription control region comprising one or several genes which are specifically activated in the mammary gland; (2) a first DNA sequence region which does not get trans- lated; (3) a DNA sequence which codes for a signal peptide which allows the secretion into the milk; (4) a DNA sequence which contains the genetic information for a heterologous peptide or a protein; and (5) a DNA sequence which contains a polyadenylation site and a transcription termination signal. There is also a recombinant vector which contains such a construct, and processes for obtaining proteins from the milk of transgenic animals in whose genome a DNA construct is integrated.

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