Dna decontamination method

Details Dna decontamination method Dna decontamination method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2576142

The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.

La présente invention a trait à un procédé pour l'amplification d'un acide nucléique cible méthylé dans un échantillon tout en évitant l'amplification d'un acide nucléique cible non méthylé par son inactivation. Cela est réalisé par une digestion d'enzyme de restriction après un traitement au bisulfite de l'acide nucléique cible. L'invention a également trait à l'utilisation d'une enzyme de restriction pour éviter l'amplification de l'acide nucléique non méthylé tout en réalisant l'amplification d'un acide nucléique cible méthylé dans un échantillon et des trousses pour la réalisation des procédés selon l'invention.

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