Dna encoding 1,3-beta-d glucan synthase subunits

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C07K 16/40 (2006.01) C12N 1/19 (2006.01) C12N 9/10 (2006.01) C12Q 1/48 (2006.01) C07K 14/395 (2006.01)

Patent

CA 2173956

DNA molecules encoding proteins involved in biosynthesis of 1,3-beta-D glucan are identified, cloned, expressed and used in in vitro assays to screen for antifungal compounds, including compounds that affect cell wall biosynthesis. The invention includes but is not limited to the purified DNA molecules, assays employing the DNA molecules, proteins encoded by the DNA molecules, cells expressing the DNA molecules and altered forms of the molecules.

La présente invention concerne des molécules d'ADN codant des protéines impliquées dans la biosynthèse du 1,3-béta-D glucanne. Ces molécules sont identifiées, clonées, exprimées et utilisées dans des dosages <u>in vitro</u> afin de détecter des composés antifongiques, y compris des composés qui affectent la biosynthèse de la paroi cellulaire. La présente invention comprend, mais sans s'y limiter, des molécules d'ADN purifié, des dosages utilisant les molécules d'ADN, des protéines codées par les molécules d'ADN, des cellules exprimant les molécules d'ADN et des formes modifiées des molécules.

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