Dna encoding a porcine complement inhibitor

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 14/705 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2224872

A DNA (pMCPcDNA) encoding a porcine complement inhibitor, the porcine complement inhibitor expressed therefrom and a method for screening the complement inhibitor. The pMCPcDNA can be obtained by preparing a cDNA library from porcine vascular endothelial cells and screening the cDNA encoding the porcine complement inhibitor. By using the pMCPcDNA, the porcine complement inhibitor can be produced with the use of gene recombination techniques. The pMCPcDNA is also useful in the analysis of the promoter region of the porcine complement inhibitor.

L'invention concerne un ADN (MCPp-ADNc) codant pour le complément de l'inhibiteur d'origine porcine, l'inhibiteur du complément exprimé par cet ADN et un procédé d'évaluation rapide de l'inhibiteur du complément. Le MCPp-ADNc peut être obtenu en préparant une collection d'ADNc à partir de cellules endothéliales vasculaires porcines et en effectuant une sélection des ADNc codant pour l'inhibiteur du complément en question. En utilisant le MCPp-ADNc, on peut produire un inhibiteur du complément en question, par des techniques de recombinaison génétiques. Le MCPp-ADNc est également utile pour l'analyse de la région du promoteur de cet inhibiteur du complément porcin.

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