Dna encoding a ras carboxyl-terminal processing protein

Details Dna encoding a ras carboxyl-terminal processing protein Dna encoding a ras carboxyl-terminal processing protein

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) A61K 39/395 (2006.01) C12N 9/50 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2304309

DNA encoding the human RAS carboxyl terminal processing protein has been cloned and characterized. The recombinant protein is capable of forming biologically active protein. The cDNA's have been expressed in recombinant host cells which produce active recombinant protein. Teh recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the enzyme activity, and modulators are identified.

On a cloné et caractérisé de l'ADN codant la protéine RAS humaine de traitement à terminaison carboxyle. La protéine de recombinaison est capable de former une protéine biologiquement active. De l'ADNc a été exprimé dans des cellules hôtes de recombinaison qui produisent des protéines de recombinaison actives. La protéine de recombinaison est également purifiée à partir des cellules hôtes de recombinaison. En outre les cellules hôtes de recombinaison sont utilisées pour définir un procédé permettant d'identifier des modulateurs de l'activité enzymatique et des modulateurs sont identifiés.

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