Dna encoding human acid-sensing ion channel bnac4 (asic4)

Details Dna encoding human acid-sensing ion channel bnac4 (asic4) Dna encoding human acid-sensing ion channel bnac4 (asic4)

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07H 21/04 (2006.01) C07K 14/00 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C12N 5/00 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2401398

DNA encoding human acid sensing ion channel BnaC4 has been cloned and characterized. The recombinant protein is capable of forming biologically active protein. The cDNA's have been expressed in recombinant host cells that produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.

Selon l'invention, un ADN codant le canal ionique humain de la sensation acide BnaC4 a été cloné et caractérisé. La protéine recombinante est capable de former une protéine biologiquement active. Les ADNc ont été exprimés dans des cellules hôtes recombinantes qui produisent ladite protéine recombinante. Par ailleurs, cette protéine recombinante est purifiée à partir des cellules hôtes recombinantes. En outre, ces cellules hôtes recombinantes permettent d'établir une méthode permettant d'identifier des modulateurs de l'activité du récepteur et, par conséquent, des modulateurs sont identifiés.

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