Dna encoding precursor of interleukin-1.beta. converting...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/57 (2006.01) A61K 31/70 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01) C07H 21/00 (2006.01) C07K 16/40 (2006.01) C12N 9/64 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2187162

A complementary DNA (cDNA) encoding full length form of ICErel-III is identified, sequenced and isolated. The cDNA is cloned into expression vectors for expression in recombinant hosts. The cDNA is useful to produce recombinant full length ICErel-III. The cDNA and the recombinant ICErel-III protein derived therefrom are useful in diagnostic kits, laboratory reagents and assays. The cDNA and the recombinant ICErel-III protein may be used to identify compounds that affect ICErel-III function, inflammation and cell apoptosis. ICErel-III function, inflammation and cell apoptosis may also be modulated by ICErel-III antisense or gene therapy.

On a identifié, séquencé et isolé un ADN complémentaire (ADNc) codant une forme entière d'ICE¿rel?-III. On a cloné l'ADNc en vecteurs d'expression pour obtenir une expression dans des hôtes de recombinaison. Cet ADNc est utile pour produire de l'ICE¿rel?-III de pleine longueur de recombinaison. L'ADNc et la protéine d'ICE¿rel?-III de recombinaison dérivée de ce dernier sont utiles dans des matériels de diagnostic, des réactifs de laboratoire et des dosages. On peut utiliser l'ADNc et la protéine d'ICE¿rel?-III de recombinaison pour identifier des composés qui interviennent sur la fonction de l'ICE¿rel?-III, l'inflammation et l'apoptose cellulaire. On peut également moduler la fonction de l'ICE¿rel?-III, l'inflammation et l'apoptose cellulaire en utilisant une molécule anti-sens complémentaire de l'ADN de codage de l'ICE¿rel?-III ou une thérapie génique.

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