C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/57 (2006.01) C12N 9/56 (2006.01)
Patent
CA 2191222
An isolated DNA encoding a keratinase is disclosed. The isolated DNA may be any of (a) isolated DNA which encodes the Bacillus licheniformis PWD-1 keratinase enzyme of Figure 1, (b) isolated DNA which hybridizes to an oligonucleotide probe, which hybridizes to the DNA of (a) above, and does not hybridize to DNA encoding the Bacillus licheniformis NCIB 6816 subtilisin Carlsberg serine protease, and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes a keratinase enzyme.
Cette invention se rapporte à un ADN isolé codant une kératinase. Cet ADN isolé peut être: soit (a) un ADN isolé qui code l'enzyme Bacillus licheniformis PWD-1 kératinase, illustrée dans la figure, soit (b) un ADN isolé qui s'hybride à une sonde oligonucléotidique, laquelle s'hybride à l'ADN (a) mentionné ci-dessus et ne s'hybride pas à l'ADN codant l'enzyme Bacillus licheniformis NCIB 6816 subtilisine Carlsberg sérine protéase; soit (c) un ADN isolé qui diffère des ADN isolés (a) et (b) mentionnés ci-dessus dans une séquence nucléotidique en raison de la dégénérescence du code génétique, et qui code une enzyme kératinase.
Lin Xiang
Miller Eric S.
Shih Jason C. H.
North Carolina State University
Sim & Mcburney
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