Dna expression systems based on alphaviruses

Details Dna expression systems based on alphaviruses Dna expression systems based on alphaviruses

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) A61K 39/12 (2006.01) C07H 21/02 (2006.01) C07K 14/16 (2006.01) C07K 14/18 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/11 (2006.01) C12N 15/87 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2098292

2098292 9210578 PCTABS00013 Efficient protein production from cloned DNA in animal cells has been hampered by the lack of suitable expression systems. The requirements of such an expression system are (1) to produce functional or immunogenic forms of protein molecules in a wide variety of animal cells, (2) high efficiency and (3) technical simplicity. The present invention is related to a technical solution to this problem. A DNA molecule encoding protein sequences is inserted into engineered variants of the cDNA of a positive stranded RNA virus genome from alphavirus which then, via RNA transcription and transfection into tissue culture cells, is used to produce either chimaeric virus particles for immunization or recombinant virus for protein production. Because of optimized conditions of transfection and the nature of the virus replication the present system combines both simplicity and safety in terms of handling, efficiency in terms of level of protein and RNA production, as well as broad host range.

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