Dna expression vectors and methods of use

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/863 (2006.01) A61K 39/21 (2006.01) C07K 14/16 (2006.01) C07K 14/47 (2006.01) C07K 16/10 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2401974

The present invention provides novel pGA constructs which are useful as vectors for the delivery of DNA vaccine inserts. The vaccine inserts can include the DNA transcripts of various virus, bacteria, parasite and/or fungi. Also described are methods of raising multi-epitope CD8 T-cell responses by administering therapeutically effective amounts of the novel pGA constructs comprising pathogen vaccine inserts followed by booster immunizations with a live vectored vaccine comprising the same vaccine inserts.

La présente invention concerne de nouvelles constructions de pGA utilisables en tant que vecteurs pour la délivrance d'inserts vaccinaux par l'ADN. Le inserts vaccinaux peuvent comporter les transcrits d'ADN de divers virus, de bactéries, de parasites et/ou de champignons. L'invention concerne également des procédés pour accroître les réponses du lymphocyte T CD8 à sites antigéniques multiples par l'administration de quantités thérapeutiquement efficaces de nouvelles constructions de pGA comportant des inserts vaccinaux pathogéniques suivie de vaccinations de rappel avec un vaccin vivant vectoriel comportant de tels inserts vaccinaux.

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