Dna extraction method for bursaphelenchus xylophilus from...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2722148

To extract and detect Bursaphelenchus xylophilus DNA without isolating Bursaphelenchus xylophilus from a piece of wood taken from a tree belonging to the genus Pinus and the like, provided are a method for extracting Bursaphelenchus xylophilus DNA from a collected piece of wood that includes Bursaphelenchus xylophilus, a LAMP primer set including primers that anneal to a specific region of Bursaphelenchus xylophilus DNA, and a method for detecting Bursaphelenchus xylophilus from a piece of wood by amplifying a DNA by a LAMP method using this primer set.

L'invention concerne un procédé d'extraction d'ADN de Bursaphelenchus xylophilus à partir de copeaux de bois dérivés d'une plante ligneuse du genre pin ou analogue, sans séparer Bursaphelenchus xylophilus des copeaux de bois, et de détection de Bursaphelenchus xylophilus. L'invention concerne donc un procédé d'extraction d'ADN pour Bursaphelenchus xylophilus à partir de copeaux de bois recueillis contenant Bursaphelenchus xylophilus, un ensemble d'amorces LAMP comprenant des amorces qui s'hybrident à une région spécifique de l'ADN de Bursaphelenchus xylophilus, et un procédé de détection de Bursaphelenchus xylophilus à partir de copeaux de bois dans lesquels l'ADN est amplifié par le procédé LAMP en utilisant l'ensemble d'amorces et détecté.

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