Dna fragment containing biotin biosynthesis gene and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/52 (2006.01) C12P 17/18 (2006.01)

Patent

CA 2252927

A DNA fragment containing a gene concerned in biotin biosynthesis and derived from a microorganism belonging to the genus Sphingomonas, a plasmid containing said DNA fragment, and a biotin-producing transformant containing said plasmid. There is provided a technique for utilizing a gene concerned in biotin biosynthesis and derived from a microorganism belonging to the genus Sphingomonas, for breeding of a biotin-producing microorganism by genetic engineering.

L'invention concerne des fragments d'ADN contenant un gène de la biotine biosynthétase provenant d'un micro-organisme appartenant au genre Sphingomonas, des plasmides contenant ces fragments d'ADN, des transformants producteurs de biotine et contenant ces plasmides, ainsi que des techniques servant à appliquer un gène, lequel provient d'un micro-organisme appartenant au genre Sphingomonas et participe à la biosynthèse de la biotine, pour élever par génie génétique des micro-organismes producteurs de biotine.

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