Dna fragment, method for producting transformant for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C12N 5/14 (2006.01)

Patent

CA 2540668

An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein of ToMV having a suppressor for a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cell, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.

Selon l'invention, un vecteur d'expression est construit par transfert d'ADNc de virus de la mosaïque de la tomate (ToMV) recombiné, dans lequel une protéine de revêtement de ToMV possédant un suppresseur de réaction de résistance à un virus a été substituée par un gène GFP, en aval d'un promoteur pouvant induire la transcription dépendante de l'hormone stéroïde. Dans une cellule BY-2 de tabac transformée obtenue par transfert dudit vecteur d'expression dans une cellule BY-2 de tabac, la transcription dépendante de l'hormone stéroïde est induite, permettant l'amplification de l'ARNm du gène GFP et l'induction de l'expression du GFP.

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