Dna isolation method

Details Dna isolation method Dna isolation method

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C07H 1/08 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2057266

DNA ISOLATION METHOD Abstract A novel method for the isolation of high molecular weight DNA from plants, yeast and bacteria using xanthate- forming compounds such as sodium/potassium ethyl xanthogenate is disclosed. The procedure does not require deproteination and yields clean DNA that is suitable for both PCR and Southern blotting. It can be utilized on a small scale without homogenizing the tissue. These features also facilitate automated screening or plant tissue samples, one of the labor-intensive techniques in molecular biology. This method is also adaptable for use in the field.

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