Dna joining method

Details Dna joining method Dna joining method

C - Chemistry, Metallurgy

07

H

195/1.26

C07H 21/04 (2006.01) C07K 14/62 (2006.01) C12N 15/00 (2006.01)

Patent

CA 1112195

ABSTRACT OF THE DISCLOSURE A method for treating DNA molecules that are to be combined in a subsequent joining reaction is disclosed, such that the frequency of joining an undesired combination is decreased and the frequency of joining a desired combination is enhanced. The method involves pretreatment of the reactant ends of the DNA to be joined to effect removal of 5' terminal phosphate groups, so that joining is prevented between pairs of reactant end groups from which the 5' terminal phosphate has been removed.

303972

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