Dna library and its use in methods of selecting and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2382541

A library is provided of DNA sequences consisting of 4N sequences, where N is greater than or equal to three, each sequence varying from the other sequences by comprising a different one of the 4N possible permutations of a DNA sequence of length N, wherein the library of DNA sequences is immobilised on a solid substrate. Preferably, each sequence occupies a discrete position, and preferably, the library is arranged in two or more sub-libraries, preferably 4N sub-libraries, wherein for any one sub-library one base in the DNA sequence of length N is defined and the other N-1 bases are randomised. The library may be used in screening methods to identify and characterise zinc fingers having specificity for particular nucleotide sequences.

L'invention concerne une bibliothèque de séquences d'ADN comportant 4?N¿ séquences avec N supérieur ou égal à trois, chaque séquence comportant une combinaison différente des 4?N¿ combinaisons possibles d'une séquence d'ADN de longueur N, et la bibliothèque de séquences d'ADN étant immobilisée sur un substrat solide. De préférence, chaque séquence occupe une position discrète, et la bibliothèque est disposée en deux ou trois sous-parties, de préférence 4N sous parties. Pour chaque sous-partie, une base dans la séquence d'ADN de longueur N est définie, et les autres N-1 bases sont rendues aléatoires. Ladite bibliothèque peut être utilisée dans des procédés de criblage pour l'identification et la caractérisation de doigts de zinc présentant une spécificité pour des séquences de nucléotides particulières.

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