Dna molecule encoding freezing tolerance proteins in gramineae

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01N 3/00 (2006.01) C07H 21/04 (2006.01) C07K 2/00 (2006.01) C07K 14/415 (2006.01) C12N 9/96 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2104142

ABSTRACT OF THE DISCLOSURE The present invention relates to three novel genes which have been isolated from cold-tolerant wheat species and which are induced by low temperature. The first gene, Wcs19, is preferentially expressed in green leaf tissues of tolerant gramineae species and requires both light and low temperature for maximal induction. The second gene, Wcs120, is induced only by low temperature. Different from the protein encoded by Wcs19, the protein encoded by Wcs120 contains two repeated domains that are highly conserved among RAB (rice abscisic acid-induced) and dehydrin families and appears to be light-independent. The Wcs120 protein does not however contain a serine-rich sequence present in RAB and dehydrin families. Finally, the present invention also relates to a third gene, Wcor410, also induced by low temperature as well as water stress and, to a lesser extent, by ABA. Its expression is light- independent. The protein encoded by this gene contains a serine-rich stretch as found in several drought induced proteins.

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